Single-Cell Sequencing: Technical Development and Applications in Neuroscience Research

• 作者: LIU Hanqing YANG Dan
• 来源期刊: 武汉大学自然科学学报（英文版）
• 学科: 生物学
• 年，卷（期）: 2020,（5）
• 所属期刊栏目: Biomedicine
• 页码范围: 369-376
• 页数: 8页
• 分类号: Q291
• 语种: 英文
• DOI: 10.19823/j.cnki.1007-1202.2020.0050

摘要

Understanding the structure and function of life requires the study of its basic constituent unit—ells.Cell bio-function depends largely on the coordinated expression of different genes.Traditional sequencing methods (such as DNA sequencing,and bulk RNA-seq) are performed on cell populations or tissue samples,in which biological differences between cells can be easily obscured by sample averaging and technical noises.Single-cell sequencing,an emerging technique in recent years,contributes extensively to understanding biological phenomena and studying the mechanism of diseases.Sequencing the genome of individual cells could reveal gene mutations,while single-cell transcriptome sequencing helps uncover the expression variability of genes,and most importantly,heterogeneous cells from the same samples.In this review,we will give an overview of the single-cell sequencing technique and its current applications in one of the most popular research fields: neuroscience research.