The Antiviral Efficacy of <i>Withania somnifera</i>(Ashwagandha) against Hepatitis C Virus Activity: <i>In Vitro</i>and <i>in Silico</i>Study

• 作者: Abdel-Rahman Zekri Ahmed Hassan Ibrahim Faraag Dina Mofed Mohamed Rahouma Ola Said Wafaa Ahmed
• 来源期刊: 微生物学(英文)
• 学科: 医学
• 关键词: ANTIVIRAL ASHWAGANDHA Hepatitis C Virus DOCKING Lymphocyte
• 年，卷（期）: 2020,（9）
• 页码范围: 463-477
• 页数: 15页
• 分类号: R51

摘要

<b>Objective:</b> Evaluation antiviral effects of <i>Withania somnifera</i> (Ashwagandha) leaf extract against HCV. <b>Methods:</b> cell proliferation was assessed using MTT assay after isolation of lymphocyte cells and treated with Ashwagandha water extract (ASH-WX) (6.25 mg/ml - 100 mg/ml). Assessment of quantitative Real-time PCR, Colony forming assay, TNF-<i>α</i> and molecular docking studies after infection of normal lymphocyte cells with 1 ml (1.5 × 10⁶ HCV) serum then incubated with ASH-WX at concentration 25 mg/ml & 50 mg/ml. <b>Results:</b> MTT assay revealed a significant increase (p < 0.001) in normal lymphocyte proliferation at all concentration’s particularity at 25 mg/ml with SI (6.06) and at 50 mg/ml with (5.8). While TNF-<i>α</i> significantly decreased following ASH-WX treatment compared with control untreated infected cells (p < 0.05). PCR results showed a marked viral load reduction after treatment by ASH-WX at concentration 25 mg/ml to 6.241 × 10³ IU/mL. Colony formation assay test revealed colony formation reduction compared to positive untreated control. Molecular docking analysis revealed good prediction of binding between Ashwagandha and NS5B and PKN2 compared to Sovaldi. <b>Conclusion: </b> ASH-WX may be a powerful antiviral against HCV infection.