Point-of-need molecular processing of biosamples using portable instrumentation to reduce turnaround time

• 作者: Kambouris Manousos E. Siamoglou Stavroula Kordou Zoe Milioni Aphroditi Vassilakis Stamatis Goudoudaki Stavroula Kritikou Stavroula Manoussopoulos Yiannis Velegraki Aristea Patrinos George P.
• 来源期刊: 生物安全与健康（英文）
• 关键词: Point-of-Need Biosurveillance Public health Biosecurity - biosafety Portable PCR Portable NGS Quarantine pathogens
• 年，卷（期）: 2020,（3）
• 所属期刊栏目: Methodology
• 页码范围: 177-182
• 页数: 6页
• 语种: 中文
• DOI: 10.1016/j.bsheal.2020.06.001

摘要

Infectious agents, both standard and emerging or engineered, might initiate outbreaks with global impact and/or existential-level threat potential. Such agents might infect human, livestock, exploitable plant cultures or the environment at large. Instead of responding by sampling teams which may also carry out prolonged surveillance, a more effective concept is to pre-process the samples, possibly to nucleic acid extract so as to transport them without biological risks by standard channels, or to process them up to a point and return the results, leaving the samples which only need specialized tests and dedicated equipment. In this context a portable device, allowing multiple routine and crisis-management applications due to its inherent flexibility, is scrutinized against alternatives and possible needs so as to develop a novel standard of surveillance and intervention, meant to facilitate containment by providing faster much more reliable laboratory results at a fraction of older generation mobile nucleic acids analyzers. Additional steps, such as agarose in pills and buffers pre-packaged in expendable syringes with pistons locked by improvised safety features may improve the Complexity Level of the whole system so as to make it appealing to intervention/crisis management personnel, while the instrument proper may double as backup for respective benchtop devices.