Cloning and Expression Analysis of TcREMORIN Gene Related to Anthracnose Resistance in Taxus chinesis

• 作者: Chen Minghui Li Yanjiao Zhang Zhilu Tong Weishuang
• 来源期刊: Plant Diseases and Pests(植物病虫害研究:英文版)
• 学科: 医学
• 关键词: Taxus chinesis ANTHRACNOSE Remorin QRT-PCR
• 年，卷（期）: 2020,（5）
• 页码范围: 24-27
• 页数: 4页
• 分类号: R73

摘要

[Objective]The paper was to obtain a pathogen-inducible responsive gene TcREMORIN from Taxus chinesis.[Method]Total RNA was extracted by Trizol reagent,and cDNA was synthesized using TaKaRa reverse transcription kit and Invitrogen M-MLV Reverse Transcriptase kit.The TcREMORIN gene was amplified by qRT-PCR.[Result]The full length of TcREMORIN was 778 bp with a 501 bp open reading frame(ORF)which encoded 166 amino acids.Bioinformatics analysis indicated that TcREMORIN possessed a conserved domain of remorin family genes and located probably in cell plasma membrane.The results of real-time quantitative PCR assay revealed that the target gene was induced by anthracnose of T.chinesis and its expression level was up-regulated by PEG,NaCl,H2O2 and 4℃stresses.[Conclusion]The TcREMORIN gene may play a critical role in plant-pathogen interaction.