A Comparative Study of Static Biofilm Formation and Antibiotic Resistant Pattern between Environmental and Clinical Isolate of <i>Pseudomonas aeruginosa</i>

• 作者: Akash Ahmed Fahareen Binta Mosharraf M. Mahboob Hossain Sara Sadia Chowdhury
• 来源期刊: 微生物学(英文)
• 学科: 医学
• 关键词: Biofilms Clinical Isolate Antibiotic Resistant
• 年，卷（期）: 2020,（12）
• 页码范围: 663-672
• 页数: 10页
• 分类号: R37

摘要

Biofilms are dense bacterial colonies, derived from microbially derived sessile community, networked within a polysaccharide matrix with a distinct architecture that has the attachment potential to both alive and abiotic surfaces. <i>Pseudomonas aeruginosa</i> is a model biofilm forming microorganism associated with remarkable morbidity and mortality rate due to emergence of antibiotic resistant pathogenic bacteria. Moreover, <i>Pseudomonas aeruginosa</i> originating from a biofilm is more resistant to a wide range of antibiotics than the planktonic bacteria. This research was planned to develop a comparative study of the biofilm production between potential, antimicrobial resistance of <i>Pseudomonas aeruginosa</i> isolated from mature environmental biofilm and clinical strain of the same species that did not derive from biofilm. It was observed that the <i>Pseudomonas aeruginosa</i> from environmental isolates were resistant to 15 prominent antibiotics, while clinical strain was comparatively resistant to only few of them. A confirmatory analysis of biofilm formation and antibiotic resistance pattern of these two groups of organisms was checked by 96-well microtiter plate and the disc diffusion method respectively. Finally, the results portrayed that the environmental strains with high drug resistance, potentially formed a considerable amount of biofilm in the period of a week whereas;clinical stains formed a negligible amount of biofilm within the same time frame.