Objective: To set up a database of three-dimensional fluorescence spectra of traditional Chinese medicines, Screening of medicinal materials by three-dimensional fluorescence spectroscopy, Methods: The paper Summarizes the three-dimensional fluorescence fingerprints, screens medicinal materials on basis of verifying the three-dimensional fluorescence spectra of the reference crude herbs of the national standard Chinese medicines, and combines with visual basic6.0 and microsoft access 2003 technology system to construct a three-dimensional fluorescence fingerprint database of TCMs. Results: the identification and quality control of traditional Chinese medicines (TCMs) was accuately and swiftly achieved. Conclusion: The relevant analysis on the operating system of database and the three-dimensional fluorescence spectrum database of TCMs indicates that this operating system can provide convenient channels for the inspection, verification and quality control of TCMs.