Molecular cloning and functional characterization of multiple ApOSCs from Andrographis paniculata

• 作者: GAO Wei GUO Juan HUANG Lu-Qi LIN Hui-Xin SU Ping WANG Jian WU Xiao-Yi YANG Jian ZHAO Huan
• 来源期刊: 中国天然药物
• 学科: 医学
• 年，卷（期）: 2020,（9）
• 所属期刊栏目: Special topics
• 页码范围: 659-665
• 页数: 7页
• 分类号: R284.3
• 语种: 英文
• DOI: 10.1016/S1875-5364(20)60004-8

摘要

Triterpenoids have been described in Andrographis paniculata.Oleanolic acid exhibits high biological activity and is widely used in the clinic,and β-sitosterol not only has good biological activity but also plays an important physiological role in plants.However,analysis of the biosynthetic pathway of triterpenoids in Andrographis paniculata has not been reported.Here,we provide the first report of the isolation and identification of nine 2,3-oxidosqualene cyclases (ApOSC3 to ApOSC11) from A.paniculata.The results showed that ApOSC4 represented a monofunctional synthase that could convert 2,3-oxidosqualene to β-amyrin.ApOSC5 as a bifunctional 2,3-oxidosqualene cyclases,could transfer 2,3-oxidosqualene to β-amyrin and α-amyrin.ApOSC6 to ApOSC8 composed the multifunctional 2,3-oxidosqualene cyclases that could convert 2,3-oxidosqualene to β-amyrin,α-amyrin and one or two undetermined triterpenoids.This study provides a better understanding of the biosynthetic pathway of triterpenoids in A.paniculata,and the discovery of multifunctional 2,3-oxidosqualene cyclases ApOSC5 to ApOSC8 of the facilitates knowledge of the compounds diversity in A.paniculata.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	WANG Jian		1	0	0.0	0.0
2	LIN Hui-Xin		1	0	0.0	0.0
3	ZHAO Huan		1	0	0.0	0.0
4	GUO Juan		1	0	0.0	0.0
5	SU Ping		1	0	0.0	0.0
6	YANG Jian		1	0	0.0	0.0
7	WU Xiao-Yi		1	0	0.0	0.0
8	HUANG Lu-Qi		1	0	0.0	0.0
9	GAO Wei		1	0	0.0	0.0