Spectral Characteristics and Quantitative Analysis of Troxerutin-Cu^2+ Complex

• 作者: Fengfeng XIE Liujun LIANG Wen LIU Yu LIN Zhenzhen PAN
• 来源期刊: 亚洲农业研究:英文版
• 学科: 数学
• 关键词: UV-VISIBLE SPECTROPHOTOMETRY TROXERUTIN CONTENT determination
• 年，卷（期）: 2020,（3）
• 页码范围: 60-63
• 页数: 4页
• 分类号: O17

摘要

[Objectives]This study aims to establish a simple,convenient,fast,low-cost,low-consumption and efficient method for quality control of troxerutin.[Methods]In NaAc-phosphate buffer at pH=8,troxerutin could react with Cu^2+to form complex,which had a characteristic absorption peak at 408 nm in the ultraviolet spectrum.The content of troxerutin could be determined indirectly by measuring the absorbance of the complex.[Results]Within the linear range of 27.82-74.19μg/mL,the absorbance of the complex has a good linear relationship with the concentration of troxerutin.The regression equation is A=0.0119C-0.1384(r=0.9991),and the detection limit is 1.59μg/mL.This method used to determine the content of troxerutin in samples from different manufacturers,and the recovery is 100.9%-102.7%.[Conclusions]The ultraviolet spectrophotometry is used in this experiment to indirectly determine the content of troxerutin by measuring the absorbance of troxerutin-Cu^2+complex,and the results are accurate,reproducible and highly precise.This method can be used for quantitative analysis of troxerutin.