The Expression and Clinical Significance of ABCG2, Oct4 and Nanog in Laryngeal Carcinoma

• 作者: Guoqing Fu Liu Xie Qingyan Han Shufang Li Xiang Wang Xiaoqin Wu Ye Nong Tan Yisha Wu
• 来源期刊: 癌症治疗(英文)
• 学科: 医学
• 关键词: Laryngeal carcinoma OCT4 NANOG ABCG2 RT-PCR Real-Time Quantitative PCR
• 年，卷（期）: 2020,（11）
• 页码范围: 660-672
• 页数: 13页
• 分类号: R73

摘要

<strong>Objective: </strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">To investigate the protein expression and clinicopathological characteristics of ABCG2</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">, </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Oct4</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> Nanog in laryngeal cancer tissues, and to seek new molecular markers for the diagnosis of laryngeal cancer.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Methods: </span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The laryngeal cancer tissues and paracancerous tissues of 87 patients with laryngeal carcinoma diagnosed in the department of otorhinolaryngology and head and neck surgery in our hospital from April 2016 to April 2018 were selected as the subjects. QRT-PCR, Real-time PcR, Western blot and immunohistochemical staining were used to detect the expression of ABCG2, Oct4 and Nanog in (Tumor Tissue) and (Adjacent Tissue) in tumor tissue and paracancerous tissue.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Results: </span></b></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">The results of RT-PCR showed that the positive rates of ABCG2, Oct4 and Nanog in laryngeal carcinoma tiss