Efficacy of decoction from Jieduan Niwan formula on rat model of acute-on-chronic liver failure induced by porcine serum

• 作者: Chen Yu Du Yuqiong Gao Lianyin Li Jinxia Zhang Qiuyun
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2020,（4）
• 所属期刊栏目: Research Articles
• 页码范围: 602-612
• 页数: 11页
• 语种: 英文

摘要

OBJECTIVE: To dynamically observe the efficacy of Jieduan Niwan formula (JDNW) on a rat model of acute-on-chronic liver failure (ACLF).METHODS: Seventy Wistar rats were divided into control group (6 rats),model group (22 rats),JDNW group (21 rats),and SP600125 group (21 rats).13 weeks' porcine serum injection followed with D-galactosamine and lipopolysaccharide joint acute attack was used to establish ACLF model.Rats in JDNW group were orally given JDNW formula for 3 days before acute attack;rats in SP600125 group were injected with SP600125 30 min ahead of acute attack.Rats were sacrificed respectively at 4,8 and 12 h after model established.Alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (TBIL),Creatinine (CR),blood urea nitrogen (BUN),prothrombin activity (PTA) were examined by biochemical process,Tumor necrosis factor-alpha (TNF-α),interleukin-1β (IL-1β),interleukin-6 (IL-6),interleukin-10 (IL-10),transformed growth factor-beta 1 (TGF-β1),High mobility group box-1 (HMGB-1),CD3,CD4,CD8 were analyzed by enzyme-linked immunosorbent assay,apoptotic index (AI) was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling staining,expression of Bad,phosphorylated Jun N-terminal kinases (p-JNK) and Cytochrome C (Cyt C) were detected by immunohistochemical analysis,Bax and Bid were detected by Western blot analysis.RESULTS;In model group,the levels of ALT,AST,TBIL,CR,BUN,IL-1β,IL-6,IL-10,TGF-β1 and HMGB-1 remarkably increased and PTA decreased compared with control group (P ＜ 0.05),as time goes on,ALT,AST,TBIL,CR,BUN,continued to grow,while IL-1β,IL-6,IL-10,HMGB-1,TGF-β1 and PTA gradually decreased;massive necrosis could be seen;the levels of TNF-α,CD3,CD4,CD8,AI,p-JNK,Bax,Bad,Bid and Cyt C increased at 4 h and peaked at 8 h,but decreased at 12 h (P ＜ 0.05).JDNW group,by contrast,showed less pathological injury,increased PTA level,and reduced ALT,AST,TBIL,TNF-α,IL-1β,IL-6,IL-10,TGF-β1,HMGB-1,CD3,CD4 and CD8 levels (P ＜ 0.05),moreover,the AI and expression of p-JNK,Bax,Bad,Bid and Cyt C were lower than model group at 4 and 8 h but were higher at 12 h (P ＜ 0.05).Similar results were observed in SP600125 group.CONCLUSION: An ACLF rat model with low mortality can be established by porcine serum joint with D-galactosamine + lipopolysaccharide induction;JDNW decoction can effectively suppress the inflammatory reaction,improve the immune system,and protect the liver of ACLF rats,the mechanism might involve the inhibition of the JNK-induced mitochondrial apoptotic pathway.