Analysis on the Expression of TaMOR Gene in Two Different Spring Wheat Varieties at Different Years

• 作者: Beixi ZHU Chaoran LI Huafang ZHU Li ZHU Lijun ZHU Meifeng FU Qianglin WANG Xia LI Xin LIU
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Spring wheat Secondary root Primary roots Root TaMOR gene
• 年，卷（期）: 2020,（4）
• 页码范围: 4-6
• 页数: 3页
• 分类号: R73

摘要

[Objectives]The TaMOR gene is a gene that affects the initiation and growth of the secondary roots of wheat,but the expression patterns in different parts of the wheat root system and the differences in expression in different varieties are not clear.This study aimed to investigate the expression of the TaMOR gene in the seminal roots,secondary roots and root base.[Methods]Real-time fluorescence quantitative PCR technology was used to analyze the relative expression levels of the TaMOR gene in seminal roots,secondary roots and root base of seedlings of ancient variety Monkhead and modern variety Longchun 35.[Results]There was no significant difference in the number of seminal roots between Longchun 35 and Monkhead,and the numbers of seminal roots of the two varieties did not change significantly during the three sampling periods.The number of secondary roots and shoot dry weight of Longchun 35 were significantly higher than those of Monkhead,and the number of secondary roots and shoot dry weight of both varieties increased with the sampling time point.The root dry weight of Monkhead increased with the sampling time,while Longchun 35 showed the largest value at the second time.The fluorescence quantitative PCR results showed that for 13-day seedlings,the relative expression of the TaMOR gene in root base was significantly higher than that in the seminal roots and secondary roots.There was no significant difference in the relative expression of gene TaMOR in the root system of Monkhead and Longchun 35.[Conclusions]The root allocation of gramineous crops decreases with the breeding years,and the difference in gene TaMOR expression level needs further study.