A mixed light field generated from a two-level atomic ensemble can be used for two photon interference.In this mixed light field,correlated paired photons generated from a four wave mixing process provide a signal of two-photon interference,while Rayleigh scattered photons of the pump laser provide a stable reference to calibrate the normalized second-order correlation function.We demonstrate two-photon interference using the Hong-Ou-Mandel and Hanbury Brown-Twiss interferometers.A direct quantitative comparison between theoretical predictions and experimental data is performed under perturbed experimental conditions,which reveal this kind of light source has potential application for quantum metrology.