Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals

• 作者: Aiming Wang Dong Zhang Heping Cheng Liangyi Chen Runlong Wu Shiyuan Chen Zichen Wang
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2020,（10）
• 所属期刊栏目: Reviews
• 页码范围: 1182-1190
• 页数: 9页
• 语种: 英文

摘要

An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system levels.The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors.In this brief review,we summarize recent advances in miniature fluorescence microscopy for neuroscience,focusing mostly on two mainstream solutions-miniature single-photon microscopy,and miniature two-photon microscopy.We discuss their technical advantages and limitations as well as unmet challenges for future improvement.Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements,long and complex protocols,and even disease progression and