Involvement of moesin phosphorylation in ischemia/reperfusion induced inner blood-retinal barrier dysfunction

• 作者: Jian Yu Jing Wu Jing Xu Ke Xiong Lin-Jiang Chen Ming Ma Qiong Liu
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: RETINAL ISCHEMIA-REPERFUSION MOESIN p38 MITOGEN-ACTIVATED protein kinase INNER blood-retinal barrier mice
• 年，卷（期）: 2020,（4）
• 页码范围: 545-551
• 页数: 7页
• 分类号: R36

摘要

AIM: To investigate the role of moesin and its underlying signal transduction in retinal vascular damage induced by retinal ischemia-reperfusion(RIR) insult.METHODS: C57 BL/6 mice were subjected to continued ischemia for 45 min, followed by blood reperfusion. The expression and phosphorylation of moesin in retinal vessels were detected by immunohistochemistry and Western blotting. The inner blood-retinal barrier was evaluated using FITCdextran leakage assay on whole-mount retina. Further studies were conducted to explore the effects of p38 mitogen-activated protein kinase(MAPK) pathway on the involvement of moesin in RIR-evoked retinal vascular hyperpermeability response. RESULTS: It revealed that RIR induced moesin phosphorylation in a time-dependent manner after reperfusion. The phosphorylation of moesin was alleviated by inhibitions of p38 MAPK, while this treatment also ameliorated the dysfunction of inner blood-retinal barrier. CONCLUSION: The results suggest that moesin is involved in RIR-evoked retinal vascular endothelial dysfunction and the phosphorylation of moesin is triggered via p38 MAPK activation.