Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling

• 作者: Beong Il Je Dae Youn Hwang Jum Soon Kang Lu Guo Woo Hong Joo Young Hoon Park Young Whan Choi
• 来源期刊: 亚太热带医药杂志（英文版）
• 年，卷（期）: 2020,（12）
• 所属期刊栏目: Original Articles
• 页码范围: 557-565
• 页数: 9页
• 语种: 英文
• DOI: 10.4103/1995-7645.296724

摘要

Objective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais.Methods: Dried mulberry leaves, dried mulberry leaves with 50％ raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25 ℃, after which the dried mulberry leaves and fermented product were extracted with 70％ ethanol and subjected to high performance liquid chromatography (HPLC). The contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid were determined. We then used the 3T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro. Results: HPLC showed that fermentation changed the contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid. Furthermore, fermented dried mulberry leaves with 50％ raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves, fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption. Additionally, fermented dried mulberry leaves with 50％ raw silkworm pupa inhibited PPAR-γ signaling.Conclusions: Fermentation with Cordyceps militaris enhanced anti-adipogenesis efficacy of mulberry leaves.