Research on Key Technology of Efficient Production of Bovine Embryos

• 作者: Bin SONG Guizhi TONG Hongguang WANG Weixia WANG Xintao LI Yongsheng HAN
• 来源期刊: 农业生物技术:英文版
• 学科: 经济
• 关键词: CATTLE Embryo bisection Blastocyst development rate
• 年，卷（期）: 2020,（4）
• 页码范围: 144-145
• 页数: 2页
• 分类号: F42

摘要

[Objectives]This study was conducted to explore the effects of three different splitting fluids on the bisection effects of bovine morulae and blastocysts.[Methods]With the help of a micromanipulator,the morulae and blastocysts that were routinely produced in the body from the 6 th to the 8 th d were divided into half-embryos in vitro to observe their development,and the half-embryos with a restored morphology and a blastocyst trophoblast were selected.[Results]The success rates of bisection of morulae in PBS+0.2 mol/L sucrose and PBS+5%PVP were significantly higher than that in PBS(P<0.05),and the values in the three liquids were 95.7%,97.2% and 50%,respectively,while the blastocyst development rates and of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).When blastocysts were bisected in PBS+0.2 mol/L sucrose and PBS+5% PVP,the success rates of bisection were significantly higher than that of PBS(P<0.05),and the values in the three liquids were 96.8%,95.5% and 50.0%,respectively,while the development rates of half-embryos and the pregnancy rates of transplantation were not significantly different(P>0.05).The development rate of half-embryos bisected from blastocysts was significantly higher than that of morulae.[Conclusions]This study improves the efficiency of bovine embryo bisection,which is beneficial to the application of embryo bisection technology in cattle breeding.