In vitro efficacy of new synthetic benzimidazole-related compounds against Schistosoma mansoni adult worms

• 作者: Amira Taman Basem Mansour Fouad El-Shehabi Magda Ayoub Menerva Tadros Samar N. El-Beshbishi Samia El-Bardicy
• 来源期刊: 亚太热带医药杂志（英文版）
• 年，卷（期）: 2020,（12）
• 所属期刊栏目: Original Articles
• 页码范围: 566-572,后插1
• 页数: 8页
• 语种: 英文
• DOI: 10.4103/1995-7645.296725

摘要

Objective: To evaluate the in vitro antischistosomal activity of two new synthetic benzimidazole-related compounds: NBTP-OH and NBTP-F. Methods: Schistosoma adult worms were recovered from mice infected with Schistosoma mansoni cercaria, washed and then incubated in the culture media with different concentrations of compounds NBTP-OH and NBTP-F up to 72 h. Scanning electron microscopy was conducted to report morphological changes. Results: Incubation of adult Schistosoma mansoni with 10 μg/mL of NBTP-OH for 48 h killed 81.25％ of worms. The calculated LC50 and LC9072 h post-incubation were 6.8 μg/mL and 9.8 μg/?mL, respectively. Exposure of worms to 10 μg/mL of NBTP-F killed 89.5％ of worms after 48 h, mostly males (83.3％), the LC50 and LC90 after 72 h of incubation were 4.8 μg/mL and 6.9 μg/mL, respectively. Worms incubated for 72 h with these compounds revealed swelling and deformity of oral sucker, disorganization and erosion of the tegument when examined with scanning electron microscopy. Conclusions: NBTP-OH and NBTP-F possess in vitro antischistosomal activities; however, in vivo studies should be conducted to examine their antischistosomal effects.