Comparative Study on the Extraction of Total RNA from Different Tissue Parts of Cone Snail(Conus geographus)

• 作者: Bingmiao GAO Chao PENG Qin CHEN Shusheng LIN
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Cone snail Venom gland RNA extraction Trizol method
• 年，卷（期）: nyswjsywb_2020,（3）
• 页码范围: 12-14
• 页数: 3页
• 分类号: R73

摘要

[Objectives] This study was conducted to compare the quality of total RNA extracted from different tissues of cone snail( Conus geographus).[Methods] The total RNA of four different tissues of cone snail,venom gland,venom tube,salivary gland and tooth gland was extracted by the Trizol method. The total RNA of cone snail was detected by agarose gel electrophoresis,NanoDrop~(TM) 2000 spectrophotometer and Aligent 2100 biological analyzer. [Results]The total RNA extracted from different tissues of cone snail showed clear band,and thus had similar concentrations and purity,and the highest yield was obtained from venom tube. [Conclusions] The total RNA extracted from different tissue parts of cone snail could meet the basic requirements of molecular biology,and its venom tube was the best tissue part for extracting total RNA,which lays a foundation for molecular biology research such as high-throughput transcriptome sequencing of cone snail.