Morphofunctional Properties of Human Platelets, Treated with Low Doses of Hydrogen Peroxide

• 作者: Maya V. Storozheva
• 来源期刊: 生物科学与医学(英文)
• 学科: 工学
• 关键词: PLATELETS GRANULES LAMELLA DEGRANULATION STABILIZATION
• 年，卷（期）: 2020,（9）
• 页码范围: 125-130
• 页数: 6页
• 分类号: TG1

摘要

We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H₂O₂). It was shown that platelet incubation with 20 - 75 <em>μ</em>M H₂O₂ during 24 hours at 20<span style="white-space:nowrap;">&deg;</span>C - 22<span style="white-space:nowrap;">&deg;</span>C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 <em>μ</em>M H₂O₂. 30 - 60 μM H₂O₂ dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H₂O₂. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.