Effect of pH, Temperature, and CO2 Concentration on Growth and Lipid Accumulation of Nannochloropsis sp. MASCC 11

• 作者: CUI Hongwu MENG Fanping PENG Xiaoling WANG Yuejie YI Xiaoyan
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2020,（5）
• 页码范围: 1183-1192
• 页数: 10页
• 语种: 英文

摘要

This study determined growth and lipid accumulation in Nannochloropsis sp. MASCC 11 cultivated at different pH, temperatures, and CO2 concentrations in 10-day period. The suitability for biodiesel production was also evaluated based on the fatty acid profiles of microalgae lipid. Nannochloropsis sp. MASCC 11 showed an excellent tolerance to acidic pH (as low as 4.0), high temperatures (at least 40℃), and high CO2 concentrations (5％-15％), which are major stressed conditions in flue gas. The highest algal biomass was acquired at pH of 9.0 (0.44gL–1), a temperature of 35℃ (0.63gL–1), and a CO2 concentration of 5％ (2.27gL–1). Maximum lipid production was obtained at pH of 6.0 (108.2mgL–1), a temperature of 35℃ (134.6mgL–1), and a CO2 concentration of 5％ (782.7mgL–1). Synthesis of polyunsaturated fatty acids (PUFAs) in biomass was stimulated under high CO2 concentrations, remaining above 80％ of total fatty acids, mainly composed of C16:3, C18:2, and C18:3. This led to the algae-based biodiesel having a lower oxidation stability, better cold flow properties, and other parameters such as its kinematic viscosity, cetane number, and spe-cific gravity complied with ASTM or EN 14214 biodiesel specifications. Therefore, the improvement of oxidative stability needs to be considered before Nannochloropsis sp. MASCC 11 lipid can be used for biodiesel production, even if this species can grow well under stressful conditions.