[Objectives] This study was conducted to understand soil p H changes during tobacco planting and its reasons. [Methods]Soil samples were collected from Jingdong,Jinggu and Zhenyuan tobacco-planting areas of Pu'er City before tobacco planting and during different growth stages of tobacco. [Results] Planting flue-cured tobacco changed the soil p H value. First,it was affected by the nicotine produced by the root system of tobacco plants. Second,it was affected by the type of soil. [Conclusions] This study has certain theoretical guiding significance for the cultivation management of flue-cured tobacco and the arrangement of ensuing crops.