Aortic heterogeneity across segments and under high fat/salt/glucose conditions at the single-cell level

• 来源期刊: 国家科学评论（英文版）
• 关键词: aorta single-cell RNA sequencing cardiovascular disease
• 年，卷（期）: 2020,（5）
• 页码范围: 881-896
• 页数: 16页
• 语种: 英文
• DOI: 10.1093/nsr/nwaa038

摘要

The aorta,with ascending,arch,thoracic and abdominal segnents,responds to the heartbeat,senses metabolites and distributes blood to all parts of the body.However,the heterogeneity across aortic segments and how metabolic pathologies change it are not known.Here,a total of 216 612 individual cells from the ascending aorta,aortic arch,and thoracic and abdominal segments of mouse aortas under normal conditions or with high blood glucose levels,high dietary salt,or high fat intake were profiled using single-cell RNA sequencing.We generated a compendium of 10 distinct cell types,mainly endothelial (EC),smooth muscle (SMC),stromal and immune cells.The distributions of the different cells and their intercommunication were influenced by the hemodynamic microenvironment across anatomical segments,and the spatial heterogeneity of ECs and SMCs may contribute to differential vascular dilation and constriction that were measured by wire myography.Importantly,the composition of aortic cells,their gene expression profiles and their regulatory intercellular networks broadly changed in response to high fat/salt/glucose conditions.Notably,the abdominal aorta showed the most dramatic changes in cellular composition,particularly involving ECs,fibroblasts and myeloid cells with cardiovascular risk factor-related regulons and gene expression networks.Our study elucidates the nature and range of aortic cell diversity,with implications for the treatment of metabolic pathologies.