Characterization of a broad substrates specificity acyl-CoA: diacylglycerol acyltransferase 1 from the green tide alga Ulva prolifera

• 作者: Chengwei Liang Dong Xu Jian Zhang Naihao Ye Xiao Fan Xiaowen Zhang Xiaoyuan Chi Yan Zhang Yitao Wang
• 来源期刊: 海洋学报（英文版）
• 年，卷（期）: 2020,（10）
• 所属期刊栏目: Articles
• 页码范围: 42-49
• 页数: 8页
• 语种: 英文

摘要

Triacylglycerols (triglycerides, TAGs) are the major carbon and energy storage forms in various organisms, and important components of cellular membranes and signaling molecules; they have essential functions in multiple physiological processes and stress regulation. Acyl-CoA: diacylglycerol acyltransferase (DGAT) catalyzes the final and only committed acylation step in the synthesis of TAGs in eukaryotes. The present work identified and isolated a novel gene, UpDGAT1, from the green tide alga Ulva prolifera. The activity of UpDGAT1 was confirmed by heterologous expression in a Saccharomyces cerevisiae TAG-deficient quadruple mutant. Results of thin-layer chromatography and BODIPY staining indicated that UpDGAT1 was able to restore TAG synthesis and lipid body formation in the yeast. Lipid analysis of yeast cells revealed that UpDGAT1 showed broad substrate specificity, accepting saturated as well as mono- and polyunsaturated acyl-CoAs as substrates. High salinity and high temperature stresses increased UpDGAT1 expression and TAG accumulation in U. prolifera. The present study provides clues to the functions of UpDGAT1 in TAG accumulation in, and stress adaptation of, U. prolifera.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	Xiaowen Zhang		2	0	0.0	0.0
2	Xiaoyuan Chi		1	0	0.0	0.0
3	Yitao Wang		2	0	0.0	0.0
4	Jian Zhang		2	0	0.0	0.0
5	Yan Zhang		2	0	0.0	0.0
6	Dong Xu		2	0	0.0	0.0
7	Xiao Fan		2	0	0.0	0.0
8	Chengwei Liang		2	0	0.0	0.0
9	Naihao Ye		2	0	0.0	0.0