Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

• 作者: Aiqing Feng Congying WANG Jianyuan YANG Jing SU Shen CHEN Wenjuan WANG Xiaoyuan ZHU
• 来源期刊: 农业生物技术:英文版
• 学科: 农学
• 关键词: Rice BLAST Resistance gene Molecular marker MAS Pi2/9 locus
• 年，卷（期）: 2020,（5）
• 页码范围: 9-12
• 页数: 4页
• 分类号: S51

摘要

Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t,designated as Pi2 SNP,Pi9 SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison method.These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitates the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes.Additionally,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of the 101 cultivars carried Piz-t,but neither Pi2 nor Pi9 band had been detected.It indicates that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t.These results provide important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.