Isolation,Identification and Susceptibility Analysis of Bacillus pyocyaners from a Canine Pyometra

• 作者: Jiande YANG Kunyuan ZHU Lan WU Yanfei LIU
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Bacillus pyocyaners Isolation and identification 16S rRNA gene Susceptibility analysis
• 年，卷（期）: 2020,（5）
• 页码范围: 94-97
• 页数: 4页
• 分类号: R97

摘要

In order to clarify the causes of the fester disease in companion animals,this study involved isolation,identification and drug susceptibility analysis of pathogenic bacteria from the uterus of an 11-year-old female dog who was admitted to the veterinary hospital of Tianjin Agricultural University.According to the results of colony morphology,smear staining microscopy,physiological and biochemical reaction and 16 S rRNA gene analysis,the bacteria was identified as a Bacillus pyocyaners.The susceptibility analysis showed that the isolate is highly sensitive to aztreonam,cefdinir,cefotaxime,cefepime,ceftazidime,ofloxacin,streptomycin,kanamycin,piperacillin and tobramycin.Moreover,the isolate is moderately sensitive to ceftriaxone,cefuroxime and cefoxitin,but resistant to ampicillin,cefazolin,cefazine and medemycin.The research results provide references for controlling infection caused by B.pyocyaners.