A stage-specific cell-manipulation platform for inducing endothelialization on demand

• 来源期刊: 国家科学评论（英文版）
• 关键词: biomaterials tissue engineering shape-memory polymer topographical conversion endothelialization
• 年，卷（期）: 2020,（3）
• 页码范围: 629-643
• 页数: 15页
• 语种: 英文
• DOI: 10.1093/nsr/nwz188

摘要

Endothelialization is of great significance for vascular remodeling,as well as for the success of implanted vascular grafts/stents in cardiovascular disease treatment.However,desirable endothelialization on synthetic biomaterials remains greatly challenging owing to extreme difficulty in offering dynamic guidance on endothelial cell (EC) functions resembling the native extracellular matrix-mediated effects.Here,we demonstrate a bilayer platform with near-infrared-triggered transformable topographies,which can alter the geometries and functions of human ECs by tunable topographical cues in a remote-controlled manner,yet cause no damage to the cell viability.The migration and the adhesion/spreading of human ECs are respectively promoted by the temporary anisotropic and permanent isotropic topographies of the platform in turn,which appropriately meet the requirements of stage-specific EC manipulation for endothelialization.In addition to the potential of promoting the development of a new generation of vascular grafts/stents enabling rapid endothelialization,this stage-specific cell-manipulation platform also holds promise in various biomedical fields,since the needs for stepwise control over different cell functions are common in wound healing and various tissue-regeneration processes.