HPLC Determination of Oleanolic Acid and Ursolic Acid in Chaenomeles cathayensis from Guizhou

• 作者: Fujun SUN Juan BAO Pengfei XIA Shimei ZHAO Zonghua ZHENG
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Chaenomeles cathayensis HPLC Oleanolic acid Ursolic acid
• 年，卷（期）: 2020,（5）
• 页码范围: 110-112
• 页数: 3页
• 分类号: R28

摘要

[Objectives]This study was conducted to establish a method for determining the contents of Chaenomeles cathayensis(Hemsl.)Schneid.,and analyze the changes in effective components of C.cathayensis grown in Guizhou,so as to provide data support for the production and quality control of C.cathayensis.[Methods]The contents of ursolic acid and oleanolic acid in C.cathayensis in different areas of Guizhou were determined.The HPLC method was adopted under following conditions:chromatographic column waters C18 column(4.6 mm×150 mm,5.0μm);mobile phase:methanol-0.1 mol/L ammonium acetate(85∶15);column temperature:25℃;detection wavelength:257 nm;flow rate 1.0 ml/min.[Results]Through methodological investigations,HPLC could be used to detect the contents of the two terpenoids oleanolic acid and ursolic acid in C.cathayensis from Guizhou.The content of oleanolic acid ranged from 0.076%to 0.144%,and the content of ursolic acid ranged from 0.201%to 0.439%.[Conclusions]A method for determining the contents of C.cathayensis was established using the HPLC method with oleanolic acid and ursolic acid as the index components.The method is accurate,reliable,and simple and easy to implement,and can serve as a reference and support for quality evaluation and standard improvement of C.cathayensis.