On the origin and continuing evolution of SARS-CoV-2

• 作者: Changcheng WU Hong Zhang Jian Lu Jie Cui Xiang Li Xiaolu Tang Xinkai Wu Xinmin Yao Yirong Wang Yuange Duan Yuhe Song Zhaohui Qian
• 来源期刊: 国家科学评论（英文版）
• 关键词: SARS-CoV-2 virus molecular evolution population genetics
• 年，卷（期）: 2020,（6）
• 页码范围: 1012-1023
• 页数: 12页
• 语种: 英文
• DOI: 10.1093/nsr/nwaa036

摘要

The SARS-CoV-2 epidemic started in late December 2019 in Wuhan,China,and has since impacted a large portion of China and raised major global concern.Herein,we investigated the extent of molecular divergence between SARS-CoV-2 and other related coronaviruses.Although we found only 4％ variability in genomic nucleotides between SARS-CoV-2 and a bat SARS-related coronavirus (SARSr-CoV;RaTG 13),the difference at neutral sites was 17％,suggesting the divergence between the two viruses is much larger than previously estimated.Our results suggest that the development of new variations in functional sites in the receptor-binding domain (RBD) of the spike seen in SARS-CoV-2 and viruses from pangolin SARSr-CoVs are likely caused by natural selection besides recombination.Population genetic analyses of 103 SARS-CoV-2 genomes indicated that these viruses had two major lineages (designated L and S),that are well defined by two different SNPs that show nearly complete linkage across the viral strains sequenced to date.We found that L lineage was more prevalent than the S lineage within the limited patient samples we examined.The implication of these evolutionary changes on disease etiology remains unclear.These findings strongly underscores the urgent need for further comprehensive studies that combine viral genomic data,with epidemiological studies of coronavirus disease 2019 (COVID-19).