Facilitation of cascade biocatalysis by artificial multi-enzyme complexes — A review

• 作者: Xinlei Wei Pingping Han Chun You
• 来源期刊: 中国化学工程学报（英文版）
• 年，卷（期）: 2020,（11）
• 所属期刊栏目: Special Topic:Biocatalysis
• 页码范围: 2799-2809
• 页数: 11页
• 语种: 英文
• DOI: 10.1016/j.cjche.2020.05.022

摘要

Multi-enzyme complexes are the results of natural evolution to facilitate cascade biocatalysis. Through enzyme colocalization within a complex, the transfer efficiency of reaction intermediates between adjacent cascade en-zymes can be promoted, resulting in enhanced overall reaction efficiency. Inspired by nature, a variety of ap-proaches have been developed for the assembly of artificial multi-enzyme complexes with different spatial organizations, aiming at improving the catalytic efficiency of enzyme cascade. A recent trend of this research area is the creation of enzyme complexes with a controllable spatial organization which helps with the mecha-nistic studies and bears the potential to further increase metabolic productivity. In this review, we summarize versatile strategies for the assembly of artificial multi-enzyme complexes, followed by an inspection of the mech-anistic studies of artificial multi-enzyme complexes for their enhancement of catalytic efficiency. Furthermore,we provide some highlighted in vivo, ex vivo, and in vitro examples that demonstrate the ability of artificial multi-enzyme complexes for enhancing the overall production efficiency of value-added compounds. Recent re-search progress has revealed the great biotechnological potential of artificial multi-enzyme complexes as a pow-erful tool for biomanufacturing.