Development of an Enteric Bacterial Enrichment Broth and Its Performance for Isolation of Clinically Significant Bacterial Pathogens from Stool

• 作者: Timothy S. Horseman Michael B. Lustik Keith S. K. Fong
• 来源期刊: 医学微生物学(英文)
• 学科: 数学
• 关键词: Enteric Bacteria ENRICHMENT STOOL Selective Agar
• 年，卷（期）: 2021,（1）
• 页码范围: 1-17
• 页数: 17页
• 分类号: O17

摘要

<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:Verdana;"> Early detection and accurate identification of foodborne pathogen outbreaks is an important public health function. Increased clinical adoption of multiplex PCR assays or culture</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">independent diagnostic tests (CIDT) correlates to more stool specimens</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">sent to public health laboratories (PHL) for characterization. Isolation and confirmation of enteric bacterial </span><span style="font-family:Verdana;">pathogens can prove difficult to consistently recover. The purpose of this study</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">was to evaluate </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">performance of a broad-use laboratory developed enrichment </span><span><span style="font-family:Verdana;">broth for isolation of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Shigella</span></i><span style="font-family:Verdana;">, and </span><i><span style="font-family:Verdana;">Yersinia</span></i> </span><span style="font-family:Verdana;">strains from stool specimens. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> The study compared differences in positivity rates among media and enrichment combinations at specific time </span><span style="font-family:Verdana;">points. Comparison of direct inoculation (DI)</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> enrichment using a</span><span style="font-family:Verdana;"> lab-developed E