Laodelphax striatellus Atg8 facilitates Rice stripe virus infection in an autophagy-independent manner

• 作者: Yuan-Ling Yu Meng-Ting Zhang Yan Huo Ji-Liang Tang Qing Liu Xiao-Ying Chen Rong-Xiang Fang Li-Li Zhang
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Special section:Insect microbiota-health and disease
• 页码范围: 315-329
• 页数: 15页
• 语种: 英文
• DOI: 10.1111/1744-7917.12771

摘要

Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant transmission.Laodelphax striatellus is the major insect vector for RSV In this study,we explored the interactions be-tween RSV infection and L.striatellus autophagy,a potential intrinsic antiviral mechanism in insects.We found that L.striatellus autophagic activity did not affect RSV infection;however,the autophagy-related-8(Atg8)gene significantly enhanced virus infection.Dur-ing RSV initial infection within the L.striatellus midgut,silencing of Atg8 expression significantly decreased the phosphorylation of c-Jun N-terminal kinase(p-JNK);however,when RSV infection is absent,silencing of Atg8 did not alter p-JNK levels.These results indicated that Atg8 might activate the JNK machinery by allowing more virus infection into cells.We further revealed that Atg8-deficiency significantly decreased RSV accumu-lation on the surface of the insect midgut epithelial cells,suggesting a receptor trafficking function of the γ-aminobutyric acid receptor-associated protein family.Using the RSV ovary entry as a model,in which vitellogenin receptor(VgR)mediates RSV cell entry,we clarified that Atg8-deficiency decreased the abundance of VgR localizing on the cytomem-brane and disturbed the attachment of RSV in the germarium zones.Collectively,these results revealed an autophagy-independent function of L.striatellus Atg8 that enhances RSV initial infection by increasing virus attachment on the infection sites.