Modulation of antiviral cytokine production and lung protection against influenza virus by the glycosides from Ligustrum purpurascens

• 作者: Chen-Yang Li Da-Hong Yao Hai-Tao Xiao Muhammad Shahid Riaz Peng Zhai Qin-Chang Zhu Xiao-Jun Zhong Xiao-Peng Hu Xun Song Zhen-Dan He
• 来源期刊: 亚洲毒理学研究
• 学科: 化学
• 关键词: Ligustrum purpurascens GLYCOSIDES CYTOKINES IMMUNOREGULATION ANTIVIRAL
• 年，卷（期）: 2021,（1）
• 页码范围: 23-31
• 页数: 9页
• 分类号: O62

摘要

Ligustrum purpurascens has been used as a traditional herb for over 2,000 years in China.This study was design to investigate the modulation of antiviral cytokines and reduction in lung inflammation of virus-infected mice by the glycosides isolated from Ligustrum purpurascens.Methods:Ligustrum purpurascens glycosides(LPG)were isolated from the leaves of Ligustrum purpurascens.Proliferation of spleen lymphocytes were investigated after LPG treatment.The in vitro and in vivo cytokine modulation of LPG was studied.Furthermore,the anti-inflammatory and antiviral activities of LPG,with the potential to reduce inflammatory lung disorders,were investigated by influenza A virus infected mice.Results:LPG could significantly promote the proliferation,and also could stimulate the production of IFN-γby spleen lymphocytes in a dose-dependent manner.IFN-γexpression level was increased significantly compared to the control and presented a dose-dependent manner in vitro.Furthermore,LPG inhibit the expression of TNF-αand IL-10,which return to normal level in the cyclophosphamide-induced mice model in vivo.Besides,the histopathological analysis indicated LPG reduced acute lung injury in mice infected with influenza virus.Conclusion:This study suggested that LPG could increase the exression of IFN-γ,immunoregulation and decrease lung inflammation of virus-infected mice.