A new species of the genus Achalinus is described based on five specimens collected from the villages of Huangjialing and Fuxi, Huangshan, Anhui, China. It ca n be morphologically differentia ted from all the other species in Achalinus except for A. spinalis and A. werneri by the presence of a dotted black streak in the middle of the subcaudal. It can be distinguished from A. spinalis in that its two a nterior temporals are in contact with eye, and A. werneri by its light brown flanks. The phylogenetic rela tionship of Achalinus was reconstructed using the mitochondrial locus of cytochrome c oxidase subunit 1 (CO1). The five new specimens form a monophyletic clade with strong support. The uncorrected p-distances between the new species and other representa tives of Achalinus range from 13.6% to 21.7%. The recognition of the new species increases the number of described Achalinus species to 14.