Silencing lncRNA CTD-2510F5.4 inhibits the proliferation, invasion and RACGAP1 expression of Huh-7 human hepatocellular carcinoma cells

• 作者: Xian-Jian Wu Yuan Lu Feng Shi An-Min Wang Ya Zhang Jian Pu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Original article
• 页码范围: 16-20
• 页数: 5页
• 语种: 英文

摘要

Objective: To study the effect of lncRNA CTD-2510F5.4 silencing on the proliferation and invasion of huh-7 human hepatocellular carcinoma cells and its possible regulatory relationship with RACGAP1. Methods: The TCGA (The Cancer Genome Atlas) database was used to analyze the expression of lncRNA CTD-2510F5.4 in liver cancer and normal tissues and the correlation with the expression of RACGAP1. CTD-2510F5.4 silencing test was performed on Huh-7 human liver cancer cells, and PCR was used to verify its silencing efficiency. CCK8, Transwell, flow cytometry were used to detect cell proliferation, invasion, and apoptosis, RT-PCR, and Western blot. Detect the expression level of RACGAP1. Results: In the TCGA database, lncRNA CTD-2510F5.4 was highly expressed in liver cancer tissues, and it was positively correlated with the expression of RACGAP1 (R=0.85). After silencing CTD-2510F5.4, the proliferation and invasion of huh-7 human liver cancer cells were inhibited, apoptosis increased, and the mRNA and protein expression of RACGAP1 were down-regulated. Conclusion: lncRNA CTD-2510F5.4 may be the oncogenic lncRNA of liver cancer, and RACGAP1 may be the target of lncRNA CTD-2510F5.4. Silencing CTD-2510F5.4 can inhibit the proliferation and invasion of huh-7 human liver cancer cells, and promote apoptosis, which may be achieved by inhibiting the expression of RACGAP1.