Grainy head signaling regulates epithelium development and ecdysis in Blattella germanica

• 作者: Zheng Zhao Liang Li Min Cheng An-Di Jing Su-Ning Liu Shi-Ming Zhu Er-Xia Du Sheng Li Yun-Xia Luan Chong-Hua Ren
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Original articles
• 页码范围: 485-494
• 页数: 10页
• 语种: 英文
• DOI: 10.1111/1744-7917.12780

摘要

The transcription factor grainy head(Grh)functions in the protection of the epithelium against the external environment by generating strongly adhesive layers,and this function is conserved in vertebrates and invertebrates.In Drosophila,the top model for holometabolous insects,Grh is necessary during embryonic development,epidermal differentiation,central nervous system specification and epithelial repair.However,the function of this gene in hemimetabolous insect epithelia remains unknown.To examine the function of Grh signaling in regulating epithelium development in Hemimetabola,we focused on the Blattella germanica epidermal layer using a gene knockdown strategy.The spatiotemporal expression pattern of BgGrh was detected,and knockdown of Bg-Grh and BgCad96ca,which provide positive feedback to BgGrh,caused severe defects in new epithelium development and impeded the molting process required to discard the old integument.Knockdown of the expression of BgGrh and BgCad96ca caused increased ex-pression of chitin synthase gene(BgCHSl)and chitinase gene(BgCht5),the upregulations of which should be mediated by the higher level of hormone receptor 3(BgHr3)gene.In conclusion,epithelium development is regulated by Grh signaling,which might represent a potential target for the control of urban pest cockroaches.