Naoxintong capsules(脑心通胶囊)modulates tumor necrosis factor-α-induced endothelial senescence through silent information regulator 1 signaling

• 作者: GUO Qianyun ZHANG Bin ZHANG Haitong ZHANG Erli WU Yongjian
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Research Articles
• 页码范围: 212-218
• 页数: 7页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.02.002

摘要

OBJECTIVE:To investigate the protective effects of Naoxintong capsules(脑心通胶囊,NXT)on tumor necrosis factor-α(TNF-α)-induced senescence inen-dothelial cells and its mechanism.METHODS:Human umbilical vascular endothelial cells(HUVECs)were treated with TNF-α±NXT and assessed for silent information regulator 1(SIRT1)expression and signaling.Cells were stained with beta-galactosidase to assess the levels of cellular se-nescence.SIRT1 was silenced through siRNA trans-fection.RESULTS:TNF-α treatment led to the downregula-tion of SIRT1,resulting in forkhead box O1(FoxO-1)acetylation,p53 acetylation and enhanced p21 ex-pression.Following TNF-α treatment,higher Saβ-Gal activity improved.TNF-α enhanced the migra-tion of HUVECs and increased SIRT1 expression,both of which were attenuated by NXT treatment.The downstream targets of SIRT1 including FoxO-1/p53/p21 were also modulated,and HUVECs were protected from TNF-α-induced senescence.In con-trast,the NXT-mediated protection was prevented by SIRT1 silencing.CONCLUSIONS:These findings suggest that sus-tained endothelial senescence can be induced by TNF-α stimulation via the SIRT1/FoxO-1/p53/p21 pathway.The protection of NXT against TNF-α was partially mediated through its effects on SIRT1.This highlights the promise of NXT as a therapeutic for atherosclerosis.