This paper is the result of an investigation of the flora and traditionalknowledge in the conception of Javaé indigenous people from the Txuirivillage located on Bananal Island, Brazil. The objective is to investigatethe plants used by these indigenous people, their diverse uses and tounderstand how traditional knowledge is passed on to new generations.This is a qualitative, descriptive and interdisciplinary survey, whose datacollection strategies included the application of semi-structured questionnairesand collection of plants for cataloguing according to AngiospermPhylogeny Group or APG III (2009). We identified 26 plant species, usedfor various purposes such as medicinal use, food, construction, craft andcultural, which were deposited in the Herbarium of the Federal Universityof Tocantins. Roots, stem and leaf are the plant parts most used bythe community. The plants mentioned were most frequently found on thebanks of the Javaés River and in the backyard of the residences. Significanttraditional knowledge of these people about the plants are transmittedto new generations, through visual, orality and experimentation. Ethnobotanicalstudies strengthen research in ecopsychology while allowingresearch into the interactions between human populations and plants.