Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant

• 作者: Haochun Xi Yongjun He Huoying Chen
• 来源期刊: 园艺学报（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Research Papers
• 页码范围: 73-80
• 页数: 8页
• 语种: 英文

摘要

Anthocyanin is abundant in a few vegetables, including eggplant. It protects plants from abiotic stress and benefits human health, making the research of anthocyanin biosynthesis increasingly important. Flowering time is an important reference for judging reproduction and adapt-ability, which can guide plant production. In this study, SmbHLH13 from eggplant was identified. Yeast one-hybrid and dual-luciferase assays showed that SmbHLH13 binded and activated the expression of structural genes SmCHS and SmF3H in anthocyanin biosynthesis and it also was bound to the promoter of the key gene SmFT in flowering. Furthermore, genetic transformation of Arabidopsis revealed that overexpression of SmbHLH13 enhanced anthocyanin accumulation and delayed flowering. These results demonstrated that SmbHLH13 might promote anthocyanin accumulation through positive regulation of SmCHS and SmF3H. Moreover, SmbHLH13 might have a role in delaying eggplant flowering.