Efficacy of Liangxue Guyuan decoction(凉血固元汤)on radiation-in-duced intestinal injury in rats via the toll-like receptor 4/myeloid dif-ferentiation primary response 88/nuclear factor-kappa B pathway

• 作者: WANG Yuguo DOU Yongqi FENG Jian XU Chengyong WANG Qian
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Research Articles
• 页码范围: 254-261
• 页数: 8页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.02.005

摘要

OBJECTIVE:To evaluate the efficacy of Liangxue Guyuan decoction(凉血固元汤,LGD)on radia-tion-induced intestinal injury in rats,and the possi-ble underlying mechanism of action.METHODS:A total of 255 male Sprague-Dawley rats were used.15 rats were assigned to the control group and the remaining 240 rats were exposed to a 60Co source at a dose of 11 Gy.Irradiated rats were randomly divided into model,dexamethasone(DXM),low-dose LGD(LGDI),and high-dose LGD(LGDh)groups and treated for 11 d.The survival rate,weight of body,intestinal pathology and the expression of toll-like receptor 4(TLR4),myeloid dif-ferentiation primary response 88(MyD88),and nu-clear factor-kappa B(NF-κB)were recorded.RESULTS:Radiation reduced the survival rate and weight of rats,destroyed the intestinal structure,in-duced an inflammatory reaction,and increased both protein and mRNA expression of TLR4,MyD88,and NF-κB in ileum.However,LGDh in-creased the survival rate,inhibited weight loss,alle-viated inflammation and improve the expression of TLR4 pathway.CONCLUSIONS:LGD increased the survival rate and inhibit weight loss of irradiated rats,and re-duced inflammation and intestinal injury.The un-derlying mechanism may involve regulation of the TLR4/MyD88/NF-KB pathway.