Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line

• 作者: QIN Yanqin CHEN Yulong ZHAO Peng FENG Suxiang WU Yaosong LIU Xuefang DONG Haoran ZHENG Wanchun MAO Xiaoning LI Jiansheng
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Research Articles
• 页码范围: 360-366
• 页数: 7页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.03.003

摘要

OBJECTIVE:To study the mechanistic effects of Tiaobu Feishen therapy(TBFS)on inflammation in-duced by cigarette smoke extract(CSE)in a human monocyte/macrophage cell line.METHODS:The human monocyte/macrophage cell line THP-1 was stimulated with 10％CSE in the presence or absence of Bufei Yishen formula(BYF),Bufei Jianpi formula(BJF)and Yiqi Zishen formula(YZF).All formulations contained serum.Pro-inflam-matory cytokines were measured in the superna-tants using enzyme-linked immunosorbent assay.The activity of STAT3 DNA binding was detected us-ing electrophoretic mobility shift assay and janus kinase/signal transducer and activator of transcrip-tion(JAK/STAT)pathway activation was assessed using Western blotting.RESULTS:The results showed that BYF,BJF and YZF treatment strongly decreased the CSE-induced se-cretion of interleukin(IL)-6,IL-8,tumor necrosis fac-tor-α and matrix metalloproteinase-9 by THP-1 cells.Furthermore,BYF,BJF and YZF treatment at-tenuated STAT3 DNA binding capacity and JAK2 and STAT3 were shown to be phosphorylated.CONCLUSION:The data revealed that BYF,BJF and YZF effectively inhibited a CSE-induced inflammato-ry response in THP-1 cells by limiting activation of the JAK2/STAT3 pathway.