Identification and activity evaluation of Astragalus Radix from different germplasm resources based on specific oligosaccharide fragments

• 作者: Shu-ying Li Di Wang Xian-rong Li Xue-mei Qin Yu-guang Du Ke Li
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Quality Evaluation of TCM
• 页码范围: 33-42
• 页数: 10页
• 语种: 英文

摘要

Objective:Based on trifluoroacetic acid(TFA)hydrolysis,polyacrylamide gel electrophoresis(PAGE)and high performance thin layer chromatography(HPTLC)analysis,the carbohydrate responsible for immunomodulatory activity are used as quality indicators for Astragalus Radix(AR).Methods:In this study,24 batches of AR from different germplasm resources were selected as the research object,and AR polysaccharides were extracted.PAGE and HPTLC methods were used to analyze the partial acid hydrolyzate of AR polysaccharides and obtain a series of saccharide fingerprints.The data were analyzed by principal component analysis to obtain the difference between AR from different germ-plasm resources.Results:The results showed that trisaccharide and tetrasaccharide could be used as differential fragments to distinguish AR of different cultivation methods;Disaccharides and trisaccharides can be used as differ-ential fragments to distinguish different species of AR.The immunological activity analysis of the specific oligosaccharide fragment of AR showed that the specific oligosaccharide fragment of AR could promote the secretion of TNF-α,IL-1β,IL-6,and NO in THP-1 cells in a concentration-dependent manner.Conclusion:Both PAGE and HPTLC methods can be used to evaluate AR from different germplasm resources.This study laid the foundation for the quality evaluation of AR medicinal herbs.