Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1 (IFIT1) in Epinephelus lanceolatus

• 作者: WANG Lei MA Teng XU Wenteng CHEN Zhangfan ZHOU Qian ZHENG Guiliang CHEN Songlin
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Life and Fisheries Sciences
• 页码范围: 383-392
• 页数: 10页
• 语种: 英文

摘要

Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), also known as interferon-induced protein 56 (IFI56) or Interferon-stimulated protein 56 (ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1 (ELIFIT1) gene was cloned for the first time. The complete cDNA of ElIFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid (AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly ex-pressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus (SKIV), ELIFIT1 gene ex- pression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.