African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

• 作者: Dan Li Yinguang Liu Xiaolan Qi Yuan Wen Pan Li Zhao Ma Yongjie Liu Haixue Zheng Zhijie Liu
• 来源期刊: 中国病毒学
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: RESEARCH ARTICLES
• 页码范围: 187-195
• 页数: 9页
• 语种: 英文

摘要

African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF),an often lethal disease in domestic and wild pigs.ASF represents a major threat to the swine industry worldwide.Currently,no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns.Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge.In the present study,a mutant ASFV strain with the deletion of ASFV MGF-110-9L (ASFV-△9L) was generated from a highly virulent ASFV CN/GS/2018 parental strain,a genotype II ASFV.Relative to the parental ASFV isolate,deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-△9L to replicate in vitro in primary swine macrophage cell cultures.The majority of animals inoculated intramuscularly with a low dose of ASFV-△9L (10 HAD50) remained clinically normal during the 21-day observational period.Three of five ASFV-△9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response,indicating partial attenuation of the ASFV-△9L strain in pigs.The findings imply the potential usefulness of the ASFV-△9L strain for further development of ASF control measures.