Hydrolyzed seawater pearl tablet modulates the immunity via at-tenuating Th1/Th2 imbalance in an immunosuppressed mouse model

• 作者: LAN Taijin LUO Xuefei MO Mingyue CHEN Zhenxing LUO Fei HAN Siyin LIU Peng LIANG Zhongxiu ZHANG Ting LI Tianyi YAN Qiangqiang LIN Yong LIN Jiang
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Research Articles
• 页码范围: 397-405
• 页数: 9页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.20210319.001

摘要

OBJECTIVE:To investigate whether Hydrolyzed Seawater Pearl tablet(HSPT)could modulate the Th1/Th2 imbalance in an immunosuppressed mouse model with Th1 to Th2 shift induced by Cy-closporine A(CsA)which can be used in the clinical treatment ofTh2 toTh1 shift diseases,and explore the possible mechanism for the adjuvant therapeu-tic efficacy of HSPT on recurrent respiratory infec-tions(RRI)and acquired immune deficiency syn-drome(AIDS).METHODS:The mice were randomly divided into six groups of five animals each,namely normal group,model group,lentinan polysaccharide tablet(LPT)group and three HPST treated groups.HPST treated groups were administered with HPST(0.51,1.02,2.04 g/kg)via intragastric gavage(i.g)for 30 consecutive days.LPT used as reference drug for positive control,LPT group was administered with LPT(8.2 mg/kg)for 30 consecutive days.Normal group and model group were received distilled wa-ter.The animals in model group,LPT group and HPST treated groups were injected intraperitoneal-ly with CsA(50 mg/kg)to establish the immunosup-pressed mice model with Th1 to Th2 shift on the 20th,22nd and 24th day,one hour after the admin-istration of the respective treatment.Animals were sacrificed one hour after the last administration to collect blood and splenic tissue.The proportion of T cells including CD8+and CD4+T cells,Th1 and Th2 in peripheral blood of experimental mice were measured by flow cytometric.The protein level in serum and mRNA level in splenic tissue of experi-mental mice for interleukin(IL)-2,IL-12,interferon-y(IFN-y),IL-4,IL-6,IL-10 and IL-13 were measured by enzyme linked immunosorbent assay and fluores-cence quantitative polymerase chain reaction re-spectively.RESULTS:HSPT elevated the proportion of T cells including both CD8+and CD4+T cells,in which the proportion of Th1 and Th2 cells increased,while the ratio of Th1/Th2 cells decreased in peripheral blood of the immunosuppressed mouse model withTh1 toTh2 shift induced by CsA.Furthermore,HSPT elevated both protein and mRNA level of Th1-type cytokines IL-2 and IFN-y,while had no sig-nificant effect on protein and mRNA level of Th1-type cytokine IL-12 and Th2-type cytokines IL-4,IL-6,IL-10,IL-13 in mouse model.CONCLUSION:Our findings suggest that HSPT can increase proportion ofT cells including both CD8+and CD4+T cells and induce Th2 to Th1 shift in both cells and cytokines,which probably was the mecha-nism to account for the adjuvant therapeutic effica-cy of HSPT on RRI and AIDS.