Optimization of Extraction Process of Chlorogenic Acid from Sambucus chinensis Lindl

• 作者: Lilan OU Pixian SHUI Ting LI Chun ZHANG
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Chlorogenic acid Ultrasonic extraction Single factor Orthogonal experiment Ultraviolet spectrophotometry
• 年，卷（期）: nyswjsywb_2021,（1）
• 页码范围: 83-86
• 页数: 4页
• 分类号: R28

摘要

[Objectives] The chlorogenic acid in Sambucus chinensis Lindl is a kind of biologically active substance with multiple pharmacological effects, but the content is relatively small. It is of great significance to find the best extraction process. Therefore, we designed single factor experiments and orthogonal experiments to screen the best conditions for extracting chlorogenic acid from S. chinensis. [Methods] With ethanol concentration, ultrasonic power, and extraction time as three single factors, single factor experiments were performed on S. chinensis, and its chlorogenic acid content was calculated from a standard curve. The contents of chlorogenic acid obtained under various conditions were compared, and then, orthogonal experiments were designed according to the orthogonal table to analyze the optimal extraction conditions of chlorogenic acid. [Results] The optimal extraction conditions of chlorogenic acid for S. chinensis were: the ethanol concentration 60%, the ultrasonic power 36 W, and the extraction time 40 min.[Conclusions] The factor that had the greatest effect on the extraction rate of chlorogenic acid in S. chinensis was ethanol concentration, followed by extraction time, and the least influencing factor was ultrasonic power. With 60% ethanol solution serving as the extractant, the ultrasonic power of 36 W, and the extraction time of 40 min, a better extraction rate was obtained. These conditions can be used as the best extraction conditions for chlorogenic acid.