Screening and Identification of Strains Converting Trans-anethole to Anisic Acid

• 作者: Jianglin YIN Xuejian LI Xueying ZHENG Yuyan FENG Jian ZHANG
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: TRANS-ANETHOLE Anisic acid Strain screening Microbial conversion
• 年，卷（期）: 2021,（1）
• 页码范围: 91-95
• 页数: 5页
• 分类号: R28

摘要

[Objectives] This study was conducted to isolate and screen the bacteria that can convert trans-anethole to anisic acid from star anise and its environmental samples, and identify the bacteria. [Methods] According to the traditional microbial culture method, with trans-anethole as the sole carbon source, through enrichment culture and separation and purification, preliminary screening by thin layer chromatography and re-screening by high-performance liquid chromatography, strains that degraded trans-anethole to produce anisic acid were obtained, and 16 S rDNA sequencing and phylogenetic tree construction were performed for genetic analysis. [Results] Eleven strains that degraded trans-anethole to produce anisic acid were obtained, among which strain NT2 that produced anisic acid with a relatively high efficiency was initially identified as Pseudomonas sp. The strain’s trans-anethole degradation rate was 45.41%, and the molar production rate and cumulative concentration of anisic acid were 21.80% and 1.96 g/L, respectively. [Conclusions] Strain NT2 has a strong ability to degrade trans-anethole to produce anisic acid, and can enrich strain resources for degradation of trans-anethole to anisic acid through microbial conversion.