Profiling of miRNAs in porcine Sertoli cells

• 来源期刊: 畜牧与生物技术杂志（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: ANIMAL GENETICS
• 页码范围: 236-249
• 页数: 14页
• 语种: 英文

摘要

Background:Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of～22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown. Methods:We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor (TNFR)-associated factor 3 (TRAF3) by ssc-miR-149. Results:The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3. Conclusion:Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.