Effects of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Un-cariae Rhynchophyllae cum Uncis)on cytochrome P450 enzyme ac-tivities in rats

• 作者: LIU Xin WANG Xinyu PENG Yinxuan WANG Xing
• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Research Articles
• 页码范围: 284-292
• 页数: 9页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.02.008

摘要

OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncari-ae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to evaluate the influence of Tianma(Rhizoma Gastrodi-ae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on the activities of CYP450 isoforms(CYP1A2,CYP3A4,CYP2E1,CYP2C19,CYP2C9,CYP2D6),which were determined by changes in the pharmacokinetic parameters of six probe drugs,theophylline,dapsone,chlorzoxazone,omeprazole,tolbutamide and dextromethorphan.Study groups included,Control group(CG),Tianma(Rhizoma Gastrodiae)group(TM),Gouteng(Ramu-lus Uncariae Rhynchophyllae cum Uncis)group(GT)and Tianma Gouteng(Gastrodia Uncaria)group(TMGT).RESULTS:No significant differences between Tian-ma(Rhizoma Gastrodiae)and control groups were found.Compared with the control group,in the Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group both the AUC and t1/2 of dapsone and tolbutamide were reduced,whereas the CL(clear-ance rate)of dapsone and tolbutamide were in-creased.Compared with the control group,in the Tianma Gouteng group,the AUC and t1/2 of dap-sone and tolbutamide were reduced,the CL of dap-sone and tolbutamide were increased,and the AUC and t1/2 of chlorzoxazone were increased and the CL of chlorzoxazone was reduced.CONCLUSION:Tianma(Rhizoma Gastrodiae)has no significant effect on the six CYP450 subtypes.The activities of CYP3A4 and CYP2C9 were increased by Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).The activities of CYP3A4 and CYP2C9 were increased,whereas the activity of CYP32E1 was re-duced by combined Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).