Collagen derived species-specific peptides for distinguishing donkey-hide gelatin (Asini Corii Colla)

• 作者: Shuo Cai Ke-xuan Zhao Meng-tong Jiang Shu-ying Han Yun-feng Zheng Xun Liu Ming Zhao Jin-ao Duan Rui Liu
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Original Articles
• 页码范围: 261-266
• 页数: 6页
• 语种: 英文

摘要

Objective:As an important food therapy product with traditional Chinese medicine (TCM) applications,donkey-hide gelatin (Asini Corii Colla,ACC) has been used for thousands of years.However,till now few effective strategy had been proposed to distinguish ACC from other animal hide gelatins,especially closely related horse-and mule-hide gelatins,which was an embarrassment of ACC quality control.Methods:Combined mass spectrometry and bioinformatic methods have been applied to identify and verify two ACC-specific peptides (Pep-1 and Pep-2) capable of distinguishing ACC from other closely related animal gelatins with high selectivity.Results:It confirmed that these two peptides could be not only used for distinguishing ACC from highly homologous horse-hide and mule-hide gelatins as well as other animal hide gelatins.Conclusion:The present study provides a simple method for species-specific peptides discovery,which can be used for assessing the quality of animal gelatin products,and ensure they are authenticable and traceable.