Altered Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/T-Cell Immunoglobulin Mucin-3 Signaling Causes the Dysregulation of Decidual CD8 +T Cells in the Third Trimester during Preeclamptic Pregnancies

• 作者: Chen Chun-Qin Wang Song-Cun Sun Feng-Run Li Meng-Die Du Mei-Rong Zhang Ying
• 来源期刊: 生殖与发育医学（英文）
• 关键词: 3 rd-trimester Carcinoembryonic Antigen-related Cell Adhesion Mol CD8 +T Cells Preeclampsia Tim-3
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Original Article
• 页码范围: 206-212
• 页数: 7页
• 语种: 中文
• DOI: 10.4103/2096-2924.325828

摘要

Objective::To investigate the frequency and function of Tim-3 +CD8 +T cells in the third trimester of normal pregnancies (NPs) and preeclamptic (PE) pregnancies. Methods::T-cell immunoglobulin mucin-3 (Tim-3) expression levels of CD8 +T cells in the decidua, peripheral blood, and umbilical cord blood obtained from women showing NPs and PE pregnancies were analyzed using flow cytometry. Decidual CD8 +T cells were cultured in the presence of recombinant human carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) protein and/or Tim-3-specific neutralizing antibodies for analyzing CD107a and intracellular cytokine expression. The placental CEACAM1 protein expression was analyzed using immunohistochemistry. Results::Tim-3 +CD8 +T cells were more abundant in the decidua than in the peripheral blood. Tim-3 expression in the decidual CD8 +T cells was significantly lower in PE patients. Decidual Tim-3 +CD8 +T cells from PE patients expressed higher levels of CD107a and the Th1-type cytokine IFN-γ, but lower levels of the Th2-type cytokine IL-4. CEACAM1 altered the CD107a, IFN-γ, and IL-4 levels; this was reversed by anti-Tim-3 antibodies. The CEACAM1 protein levels were lower in the placental tissues of women with PE pregnancies than in those of women with NPs. Conclusions::Abnormal CEACAM1/Tim-3 regulation may participate in the development of PE, accompanied by disturbed Th2 cell predominance and higher cytotoxicity of decidual CD8 +T cells.